【摘要】 作者采用黄疸出血群赖型017株钩端螺旋体（钩体）外膜免疫BALB/c小鼠,通过细胞融合等技术,获得单克隆抗体E4B7D5。亚类鉴定为IgG₁,凝集效价为1:25 600。被动免疫保护试验证明:将单克隆抗体E4B7D5腹水稀释100倍,给实验组豚鼠每只每天腹腔注射1ml,连续6天,能使豚鼠在接受同型017株钩体2ml（每毫升1×10³条）的攻击后,存活率达80％,且存活20天的豚鼠处死解剖,未见任何病变;3个对照组的存活率分别为10％,20％和10％,该三组仅有4只存活20天,经处死解剖,其中 3只发现较多的陈旧性肺出血。作者认为,单克隆抗体E4B7D5对同型017株钩体有免疫保护作用。更多还原

【Abstract】 BALB/c mice were immunized intra-peritoneally with outer envelopes ofserogroup icterohaemorrhagiae lai serovarstrain 017 leptospires. Monoclonal anti-bodies against outer envelopes （IgG₁, ag-glutinating titre 1:25 600） were producedby hybridoma technique. The monoclonalantibodies ascites （diluted 1:100） 1 mladministered intraperitoneally 1 hour be-fore the intraperitoneal injection of 2×10^?leptospires of strain 017 and the subse-quent daily administration of McAb insimilar doses for five days protected 80％of guinea pigs. Survival rates of threecontrol groups which received physiolo-gical saline, ascites of BALB/c mousemyeloma cell lines SP 2/0, and monoclonalantibodies against Pseudomonas aeruginosain plaee of monoclonal antibodies againstouter envelopes of strain 017 leptospireswere 10％, 20％ and 10％ respectively.When killed 20 days after challenge,guinea pigs of experinent group werenormal at autopsy. Old pulmonary haem-orrhages were present in the animals ofthree control groups. Passive immunopro-tection experiments have demonstratedimmunoprotection of monoclonal antibod-ies against outer envelopes of strain 017leptospires. It will be valuable for sepa-rating protective antigen fraction of outerenvelopes and studying new vaccine ofleptospira.更多还原