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兔中性粒细胞抗菌肽的纯化和鉴定

Purification and Identification of Antimicrobial Peptides of Rabbit Neutrophils

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【作者】 吴琦王伯瑶王偕秀戴保民江之泉陈俊杰

【Author】 Wu Qi;Wang Boyao;Wang Xiexiu;Dai Baomin;Jiang Zhiquan;Chen Junjie Research Unit of Leptospirosis Research Unit of Immunology Department of Biochemistry

【机构】 华西医大钩端螺旋体病研究室华西医大免疫学研究室华西医大基础医学院生物化学教研室

【摘要】 作者用0.01 mol/L柠檬酸提取家兔中性粒细胞溶酶体可溶性成份,经制备性聚丙烯酰胺凝胶电泳,从提取物中纯化出5种肽(NP1、NP2、NP3、NP4、NP5),它们的分子量在2500~6000之间,体外杀白色念珠菌试验证实,NP1、NP2有高效杀菌活性,NP3也有较强的杀菌活性。

【Abstract】 Five antimicrobial peptides were extrac-ted from the cytoplasmic granules of rabbitneutrophils with 0.01 mol/L citric acid.SDS-PAGE gel densitometric analysis of thecitric acid extract showed that they ac-counted for 34%-42% of the total extractproteins. When subjected to acid urea poly-acrylamide gel electrophoresis, five peptidesmigrated much further to the cathodethan lysozyme. Beginning with the mostcathodal component, they were in turnreferred to as NP1, NP2, NP3, NP4, andNP5. They were purified from the citricacid extract by virtue of preparative poly-acrylamide gel electrophoresis.Each of themwas of low molecular weight (2500-6000)asdetermined by SDS-PAGE. Three of the peptides were tested invitro for fungicidal activity. When Candidaalbicans (107/ml) were incubated with 12.5μg/ml of NP1 or NP2 at 37℃ for 2 h, thedeath rates of the organisms were 96.6%and 93.3% respectively. NP3 was shown tobe active against Candida albicans althoughless potent than NP1 and NP2.

【关键词】 中性粒细胞抗菌肽制备电泳
【Key words】 NeutrophilAntimicrobial peptidePAGERabbit
【基金】 国家自然科学基金
  • 【被引频次】10
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