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银杏茎段的组织培养

TISSUE CULTRUE OF STEM SEGMENTS OF GINKGO BILOBA LINN

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【作者】 罗紫娟

【Author】 Luo Zijuan(Department of Biology)

【机构】 杭州师范学院生物系

【摘要】 取自银杏(Ginkgo biloba Linn)雌雄株上当年抽出的幼嫩茎梢,剪去叶子,把嫩茎切成约0.5—0.8cm的具节小段,分别接种在十种培养基上。3天后切口处膨大;7—10天后,出现愈伤组织,节上的腋芽开始生长;30天左右培养于N6+MS微量+kH2PO4100+2.4-D3+BA1+NAA1PPm的培养基上的部分腋芽基部长出根,形成完整的再生植株。

【Abstract】 The young stem tips of male and female Ginkge biloba Linn, were cut into 0.5-0.8cm stem segments and cultivated in ten different kinds of the culture medium. Callus were induced to form in 7-10 days. The axillary buds on the noded segments with nodes began to grow. Then in the culture medium: Ne+MStrace+KH2PO4100 + 2,4-D 3 +BA1+NAA1 PPm, roots were fromed from the bases of the axillary buds in about 30 days. These buds grew up to form complete plants.

  • 【文献出处】 杭州师范学院学报(自然科学版) ,Journal of Hangzhou Teachers College , 编辑部邮箱 ,1990年03期
  • 【被引频次】9
  • 【下载频次】167
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