【摘要】 用碘化还原法将溴脱氧尿嘧啶（BudR）与牛r—球蛋白（BGG）偶联作为抗原（BudR—BGG）免疫BALB/c小鼠,取脾细胞与小鼠骨髓瘤细胞（653）进行融合,经放射免疫分析法（RIA）筛选和克隆化后,获得四株阳性杂交瘤Ⅱ₁B₇、Ⅱ₄B₄、Ⅰ₃F₁₁和Ⅱ₁H₇。杂交瘤细胞分别腹腔注射BALB/c小鼠,其中三株（Ⅱ₁B₇、Ⅱ₄B₄和Ⅱ₁H₇）获得腹水,纯化抗体并对其特性进行鉴定,三株单抗均可与BudR、IudR反应,而不与CdR、TdR及FudR反应,具有较高的特异性。更多还原

【Abstract】 Preparation and characteristics of three mouse monoclonal antibodies specific for 5-bromod-eoxyuridine(BudR)is presented. Three hybridomas, Ⅱ 1H7, Ⅱ 4B4 Ⅱ1B7,were derived from the fusion of spleencells from BALB/c mouse immunized with BudR bovine gamma globulin (BGG)conjugate and "653 mouse myeloma"cells. Enzyme-linked immunohistochemistry against whole cells labeled with BudR and radioimmunologic assay(RIA)showed that:all. three antibodies have similar binding affinities and specifities. The antibodies react to BudR specifically but do not recognize CdR,FudR or TdR.更多还原