【摘要】 水稻种子在极低温(-196℃)条件下进行(137)~Csγ射线辐照,然后进行热冲击(65℃)后处理。结果表明,极低温加热冲击处理的辐射防护效应大于极低温处理。在10—50krad剂量范围内,水稻主要性状的突变率,极低温加热冲击组及热冲击组大于常温组,而常温组又大于极低温组;在70—90krad范围内,极低温加热冲击组大于极低温组,其余两组全部死亡。各处理组在最适宜诱变剂量下的突变频率:极低温加热冲击组最高,常温组最低,其余两组居中。更多还原

【Abstract】 Dormant seeds of rice (Oryza sativa L.) variety Erjiufeng were irradiated with various dose of 137Cs gamma rays at-196℃ and immediately followed by soaking in hot water (65℃) for 2 minutes. The damage on vitality index, seedling height, seedling survival rate, seed-setting rate and micronucleus rate in the MI generation was found to be normal temperature group > heat shock group > extremely low temperature group > extremely low temperature and heat shock group; and the total mutation frequency in the M2 generation was: extremely low temperature and heat shock group > heat shock group > normal temperature group > extremely low temperature group. It is indicated from the experimental results that the extremely low temperature and heat shock treatment is effective for enhancing mutation frequency.更多还原