节点文献

A PIXE METHOD TO STUDY BIOLOGY AT THE DNA LEVEL

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具，请取消加速工具后下载。

【作者】 孙永年； 曾宪周； 汪训明； 黄祖恩； 邬显慷； 杨福家； 谈家祯；

【Author】 Sun Yongnian , Zeng Xianzhou Wang XunmingHuang Zuen Wu Xiankang Yang Fujiaand C.C.Tan(Fudan University, Shanghai 200433, China)

【机构】 Fudan University Shanghai 200433 China； Fudan University Shanghai 200433 China；

【摘要】 <正> An external proton beam system with a detection sensitivity of 10 g for ruthenium was used to study DNA. A metal- organic compound tris (4,7- diphenylphenanthroline) ruthenium (Ⅱ) [Ru(DiP)32-] was chosen as a metal marker. The target DNA labeled with Ru (DiP)32- was electrophoresed in an agarose gel and then was analysed by PIXE. The DNA sample with the metal marker showed clearly Ru peaks in the PIXE spectra, while the control showed no Ru peak at all. This method can be used to study biology at the DNA level.

【Abstract】 An external proton beam system with a detection sensitivity of 10 g for ruthenium was used to study DNA. A metal- organic compound tris (4,7- diphenylphenanthroline) ruthenium (Ⅱ) [Ru(DiP)32-] was chosen as a metal marker. The target DNA labeled with Ru (DiP)32- was electrophoresed in an agarose gel and then was analysed by PIXE. The DNA sample with the metal marker showed clearly Ru peaks in the PIXE spectra, while the control showed no Ru peak at all. This method can be used to study biology at the DNA level.

【关键词】 Deoxyribonucleic acid (DNA) Tris (4； 7- diphenylphenanthroline)ruthenium (Ⅱ) Electrophorese PIXE；
【Key words】 Deoxyribonucleic acid (DNA) Tris (4； 7- diphenylphenanthroline)ruthenium (Ⅱ) Electrophorese PIXE；
【基金】 This work was partially supported by the National Natural Science Foundation of China under grant No.18 60 122
  • 【文献出处】 Nuclear Science and Techniques ,核科学与技术(英文版) , 编辑部邮箱 ,1990年04期
  • 【下载频次】23
知网节下载
节点文献中： 

本文链接的文献网络图示:

本文的引文网络
二级参考文献(0)
参考文献(0)
共引文献(0)
节点文献
同被引文献(0)
引证文献(0)
二级引证文献(0)