【摘要】 用聚丙烯酰胺凝胶(PAG)碎块吸收洗脱法,分别将腺病毒5型(AdV-5)感染的HeLa细胞培养液、疱疹病毒2型(HSV-Ⅱ)感染的原代乳兔肾(BRK)细胞和Vero细胞培养液浓缩100倍左右,其感染性病毒的回收率依次分别为78.5％、7％和1.6％。表明该法在浓缩腺病毒中影响病毒感染性较小,有实用价值;而对HSV-Ⅱ的感染性影响较大,其原因不清。更多还原

【Abstract】 ABSTRACT Using polyacrylamine gel(PAG) debris adsorption and elution method, the HeLa cell culture fluid infected with Adeno Virus type 5(AdV-5) and the primary baby rabbit kidney cell and Vero cell culture fluids infected with Herpes Simplex Virus type 2 (HSV—Ⅱ) were concentrated about 100 times. The total recovery rates of the viruses were 78. 5%, 7% and 1. 6%, respectively. The results showed that PAG method affects the infectivity of virus slightly during AdV-5 concentration and it may be valuable for practical use. As to HSV—Ⅱ, the method affects the infectivity greatly and the cause is not clear.更多还原