【摘要】 聚丙烯酰胺凝胶盘状电泳法检测 CSF 寡克隆 IgG 区带已作为反映中枢神经系统体液免疫异常的定性指标。聚丙烯酰胺凝胶盘状电泳方法作 CSF 寡克隆 IgG 区带分折分辨力高,不需要浓缩 CSF,只要200μg 蛋白的 CSF 就可灵敏地反映出γ-球蛋白质和量的变化.作者应用本方法对124例 CSF 标本检测的结果表明:该法操作简便、灵敏度高,具有一定的临床实用价值。更多还原

【Abstract】 The polyaciylamide gel discoid shape elutrophoresis detecting CSF oligoclonal IgG bands has been served as a qualitative index which reflects the abnormality of the CNS humoral immunity. The method has an electric charge effect,a concentrating effect as well as molecular sieve effect and so on. There- fore it has high resolving power and has no need for concentrating CSF. Only 200μg protein CSF relects sensitively the changes in the quantity and quality of the γ-globulins. Especially,it can display clearly oligoclonal IgG bands. Authors have used the method to detect the CSF samples of 124 cases and the results showed that the method can be of high sensitivity and have certain clinical practical val- ue.更多还原