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干旱对不同抗旱性小麦幼苗超氧物歧化酶的影响

The Effect of Drought on Superoxide Dismutase in Seedlings of Wheat Cultivars with Different Drought Resistance

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【作者】 崔四平; 李俊明; 魏建昆; 张敬贤;

【Author】 Zhang Jingxian Cui Siping Li Junming Wei Jiankun(Institute of Agro-Physics, Physiology and Biochemistry, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang, 050051)

【机构】 河北省农林科学院农业物理生理生化研究所; 河北省农林科学院农业物理生理生化研究所 石家庄050051; 石家庄050051; 石家庄050051;

【摘要】 不同抗旱性小麦幼苗的超氧物歧化酶(SOD)活性在细胞内分布存在差异,表现在抗旱品种的叶绿体和细胞溶质SOD活性、植株总SOD活性高于不抗旱品种,而线粒体SOD活性低于不抗旱品种.干旱处理后,SOD活性增加,抗旱品种酶活性增加值高于不抗旱品种.本研究还观察到8条SOD同工酶酶谱,其中干旱诱导胚芽细胞溶质产生一条新酶谱.

【Abstract】 The results indicated that the distribution of SOD attivity varied with organelles. For chloroplasts (1000 × g precipitate fraction) and cytoplasmic fraction (11000 ×g supernatant) the drought resistant cultivar had higher SOD activity than the drought sensitive one. But for mitochondria (11000 × g pellet fraction) , the drought sensitive cultivar had higher SOD activity. Under the drought condition, total SOD activity increased greatly in the drought resistant cultivar compared to the drought sensitive one. Polyacryla-mide gel electrophoresis showed eight SOD isoenzyme bands for wheat seedlings. A new isoenzyme band was induced by water stress in the cytoplasmic fraction of the coleoptiles (including first leaves) .

【关键词】 干旱; 小麦; 超氧物歧化酶;
【Key words】 Drought; Wheat; Superoxide dismutase;
  • 【文献出处】 华北农学报 ,Acta Agriculturae Boreali—Sinica , 编辑部邮箱 ,1990年03期
  • 【被引频次】38
  • 【下载频次】122
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