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检测腺病毒早期蛋白抗体诊断婴幼儿腺病毒肺炎的初步探讨
Investigation for the Early Diagnosis of Child Adenovirus Pneumonia by Detection of the Antibody to Human Aderovinus Early Antigen
【摘要】 本试验用鼻咽拭子作病毒分离培养、CF检测AdV的抗体增长情况与IP检测Ad4V-EA-IgG等三种方法观察了69例病毒性肺炎患儿的105份血清标本。结果观察到Ip与经典判断方法的总符合率为95%,且单价血清即有诊断价值。此外,尚观察到IP检出时间较CF为早,唯(?)6个月的患者则出现较晚。AdV所致肺炎的感染率为44.9%(31/69),(?)6个月患者占患者总数的29%,均与国内报道相接近。指出设置正常细胞对照的必要性。此外,IP具有特异、灵敏、操作简单、快速与不需昂贵仪器等优点。
【Abstract】 From 69 pts. with viral pneumonia 105 ser were tested by CF for detecting the antinody to Adv & by IP for detecting Ad4V-EA-IgG. Nasal Swabs were collected for isolating AdV from these pts. Simultaneously. Among the 69 pts. 13 pts. were diagnosed as the acute AdV infection classically. In which 12 pts can be diagnosed by Ip, no false-positive result by IP in 7pts. who were exlcuded with Adv infection. So the agreement rate is 95% (19/20). For time of, detecting the IP is earlier than CF, but in pts. under 6 month the EA-IgG can be detected later. In Ip, 34.2% sera were showed a few positive cells (1-6 positive cells/200 cells) when reacted with uninfected control cells, therefore the normal control cell should be set up in order to prevent false positive result. It is concluded that the Ip technique to detect Ad4V-EA-IgG is a specific, sensitive, simple and rapid method for early diagnosis of Adv pnemonia, and needs no expensive instrument.
- 【文献出处】 贵阳医学院学报 ,Journl of Guiyang Medical College , 编辑部邮箱 ,1990年03期
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