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用1640液培养绿脓杆菌L型的研究

Study on L forms of Pseudomonas Aeruginosa Subcultured in 1640 Liquid Medium

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【作者】 王和徐英

【Author】 Wang He, et al Department of Microbiol

【机构】 贵阳医学院微生物学教研室贵阳医学院微生物学教研室

【摘要】 绿脓杆菌L型可在非高渗透性无抗生素的1640液中传代培养。其形态为圆球或长丝状,丧失鞭毛,不运动,可通过0.3μm孔径的滤菌器,酶活性和对抗生素的敏感性不同于其亲代细菌,并可进入HeLa细胞内和致细胞病变。

【Abstract】 L forms of pseudomonas aeruginosa could be subcultured in non-high osmotic 1640 liquid medium without antibiotic. They were spheroid an d elongate bodies, lost the flagellum and could not move. The L forms were passable to filters of 0.3μm pore size and different from the parent bacteria from which they were derived on the activities of enzymes and the susceptibility to antibiotics. The L forms of P. aeruginosa were within HeLa cells and cytopathic on cultures of the cells.

【关键词】 绿脓杆菌L型培养基
【Key words】 pseudomonas aeruginosaL formculture media
  • 【文献出处】 贵阳医学院学报 ,Journl of Guiyang Medical College , 编辑部邮箱 ,1990年01期
  • 【被引频次】1
  • 【下载频次】29
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