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间接荧光抗体试验诊断阿米巴病
Diagnosis of amoebiasis by indirect fluorescent antibody test
【摘要】 用生长在LAS双相培养基上48h的贴壁Entamoeba histolytica作抗原和稍加修改的Содовьев等(1971)方法进行间接荧光抗体试验,结果4例阿米巴肝脓肿病人血清全部阳性,而且阳性滴度相当高;16例急性阿米巴痢疾患者血清有13例阳性,阳性率为81%;5例正常人血清均阴性。
【Abstract】 Indirect fluorescent antibody test ( IFAT ) was carried out with slightly modified methods of Cooe(1971)for diagnosis of amoebiasis. The antigen for testing was prepared from strain G2 of EntamOeba histolytica which was collected from tube wall of 48 hours’ growing in LAS diphasic medium. The serum in IFAT was positive in all of the 4 cases of amebic liver abscess, and the positive reactions were very high. Of 16 cases of acute amebic dysentery, 13 were positive. The positive rate was 81%. Five healthy Persons were all negative
【关键词】 荧光抗体技术/方法;
痢疾;
阿米巴性/诊断;
肝脓肿;
【Key words】 Fluorescent antibody technic / methods; Dysentery, Amebic/diagnosis; Liver abscess, Amebic/diagnosis;
【Key words】 Fluorescent antibody technic / methods; Dysentery, Amebic/diagnosis; Liver abscess, Amebic/diagnosis;
- 【文献出处】 赣南医学院学报 ,Journal of Gannan Medical College , 编辑部邮箱 ,1990年04期
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