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根据Y-染色体特异DNA序列鉴定牙齿性别
SEX IDENTIFICATION OF THE DENTAL PULP BY DETECTING Y-CHROMOSOME SPECIFIC DNA SEQUENCE
【摘要】 人类牙齿的性别鉴定,是法医学个体识别中的一个重要部分。作者们应用限制性内切酶 HaeⅢ、Sau3A 水解60颗牙髓 DNA,琼脂糖凝胶电泳分离酶解片段,在紫外灯下直接观察。根据 Y-染色体特异 DNA 序列,3.4kb 长片段的有无,判断牙齿的性别。为牙齿性别的法医学鉴定提供了一种简单、准确的新方法。
【Abstract】 Sex determination of human tooth is one of the most important parts in theindividual identification in forensic medicine.The dental pulp DNA Was dige-sted with restriction donuclease and electrophoresed on agarose gel.The sexwas determined according to whether the Y-chromosome specific DNA sepuence(3.4kb long fragment)existed or not by directly observing UV light.It prov-ides a new simple method in the individual identification in forensic medicine.
【关键词】 Y-染色体特异 DNA 序列;
荧光区带;
牙髓;
【Key words】 Y-chromosme; specific; DNA; sequence; Flouresence; band; Dental; pulp;
【Key words】 Y-chromosme; specific; DNA; sequence; Flouresence; band; Dental; pulp;
- 【文献出处】 中国法医学杂志 ,Chinese Journal of Forensic Medicine , 编辑部邮箱 ,1990年03期
- 【被引频次】11
- 【下载频次】80