【摘要】 本文经实验研究,建立了以1,1′-联-(2-萘酚)作为内标监控检测生物材料中立克命的反相HPLC 方法。经实验证明空白兔肝中添加回收率达90%,同一染毒兔肝多次测定结果表明有较满意的重现性。生物材料中的内源性杂质不干扰测定。本法可用于分析毒理学的研究,也可用于立克命中毒案件的检验。更多还原

【Abstract】 A simple,reliable RPHPLC method on ODS column with UV detector for deter-mination of the anticoagulant rodenticide coumatetralyl in animal tissue was developed.1,1′-Bi-(2-Naphthol)was used as an internal standard to check the process ofexperiments.The mean recoveries from the spiked rabbit liver were around 90% atthe levels of 0.4-16mg/kg.The coeffecint of variation of 5 determination was 3.2%.The results showed that this method has a satisfactory reproducibility.The methodis practical for examining poisons in poisoning cases.更多还原