【摘要】 本文分别用四种CREST综合症硬皮病病人自发抗着丝点血清,对六种类型的细胞进行了间接免疫荧光染色。染色结果表明:这种着丝点抗原在进化中保持了高度的保守性,它在哺乳类、爬行类、两栖类、鱼类、无脊椎动物昆虫纲甚至植物细胞中都具有相似的抗原性。另外,本文对这种着丝点抗原的性质也进行了研究,细胞化学分析表明:人喉癌细胞Hep-2和中华大蟾蜍骨髓细胞内的这种着丝点抗原,它们具有完全相似的抗原性质,均为紧密结合的非组蛋白。本文中所用的实验方法在临床上可直接应用于抗着丝点抗体的检测,因此,具有一定的实践意义。更多还原

【Abstract】 Six types of cells have been studied by means of indirect immunofluores-cence staining method, using four kinds of human CREST scltroderma autoimmune antikinelochore scrum which were separately taken from Shanghai, Beijing, the United States of America. The six types of cells are Hep-2 cell strain from human laryngeal carcinoma, the bone marrow cells of Gekko swinhonis, ihe bone marrow cells of Bufo bufo, CIK cell line from the kidmey of Ctccop-haryngodon idellus, C6/36 cell strain of Aedes albopictus, the root apical cells of Allium cepa L. The four kinds of serum were found to display discrete spots in all six types of cells by immunofluorcscence. The number of spots per cell corresponds to the chromosome number in the cell types observed. In Hep-2 cells, the spots only exist in the kinetochore region of mitotic chromosomes, and each chromosome has two fluorescent spots. The results reveal that the kinetochore antigen preserves high conservation in evolution, and it possesses similar antigenicity in mammalian, reptilian, amphibian, fish, insectan and plant cells. The significance of the extensive conservation have been also discussed. In addition, the nature of the kinetochore antigen has been studied. The results of cytochemical analysis show that the antigen possesses complete similar anligenic nature in Hep-2 cells and the bone marrow cells of Bufo bufo. The kinetochore antigen is tightly bound non-histones. Its site(s) sensitive to protcslytic enzymes is (are) not exposed to outside, it is not complexed with DNA or RNA and is not extracted by 4.M Urea/2M NaCl, it is risistant to 0.1M HC1, 0.07M NaOH, but is partly destroyed by acetic acid. Finally, the methods taken in this study may be directly used to detect antikinctochore antibody in clinical diagnosis, so the study have some practical significance.更多还原