【摘要】 本工作用小鼠肝细胞膜、线粒体膜和微粒体膜为研究对象,观察经过生物活性鉴定过的肝生长刺激因子（HSS）对 CCl₄损伤膜流动性的效应。利用荧光探测剂 DPH 与含膜片液混合,测其荧光偏振度并计算膜的微粘度,以作为膜流动性改变的指标。结果表明,HSS 可减弱 CCl₄对肝细胞膜、线粒体膜和微粒体膜的损伤,使它们保持正常的流动性和稳定性。我们推测这可能是 HSS 保护肝脏的机制之一。更多还原

【Abstract】 The work studied the HSS protection on CCl₄-damaged membrane ofhepatic cytoplasma,mitochondria and microsome.Preparing membraneby means of the graded centrifugation,we labelled these membranelipid with the fluorenscent agent（DPH）to determine the fluorenscentpolarization（P）and microviscosity（η）.These were taken as an indexof membrane fluidity.The results showed that HSS had significantsuppressive effect on CCl₄-induced increase of P and η values,resultingin maintaining these two data at the control level.Since the membranefluidity is closely related with the membrane stability,the resultantincrease of membrane fluidity in HSS-treated mice may be one of itsprotective mechanisms against CCl₄ injury.更多还原