【摘要】 分离检测脑内递质氨基酸的含量,是研究脑代谢和脑功能活动的一个重要指标。本文应用高效液相色谱——柱后衍生法对大鼠脑内三个部位游离氨基酸进行了分离并对GABA(γ—氨基丁酸)、Tau(牛磺酸)、Gly(甘氨酸)、Glu(谷氨酸)和ASP(天冬氨酸)进行了定量。结果表明:随年龄增长,脑内抑制性递质GABA,Tau和Gly的含量降低或不变,兴奋性递质Glu和ASP含量增高或不变。并且还讨论了不同脑区GABA/Glu以及抑制性递质氨基酸总量与兴奋性递质氨基酸总量比值。更多还原

【Abstract】 Isolation and assay of amino-acid transmitter content in different locations in the brain is an important index for studying cerebral metabolism and functions.Isolation of five amino-acids in 3 different locations in the rat brain was performed with high performance liquid chromatography postcolum-nar derivative method.The contents of GABA, Tau, Gly, Glu and Asp were determined quantitatively.lt was found that the inhibitory transmitters such as GABA, Tau and Gly were decreasing or unchanged, while the excitatory transmitters such as Glu and Asp were increasing or unchanged with increase in age.The ratios of GABA to Glu and of the total content of inhibitory amino-acid transmitters to that of the excitatory ones in different cerebral locations were also decreased with aging.更多还原