【摘要】 采用杂交瘤技术建立了4株分泌抗大麦条纹花叶病毒新疆株系（BSMV-Xj）单克隆抗体的细胞株,分别命名为2A₂、2B₃、7D₁、7D₇,经ELISA法测定亚类,其中单克隆抗体MA-2A₂、MA2B₃属IgG2b亚类,MA7D₁属IgG_2a亚类,MA7D₇属IgG₁亚类。ELISA测定MA-2A₂、MA2B₃腹水效价为10³,MA7D₁,MA7D₇腹水效价为10⁷,4种单抗与BSMV-Xj有较强的反应,而与BSMV美国株系（BSMV-Am）仅有微弱反应。BSMV-Xj经不同条件处理后,与4种单抗ELISA反应值均有不同程度的下降。更多还原

【Abstract】 Four hybridoma cell lines designated as 2A2, 2B3, 7D1,7D7,that secreted monoclonal antibodies against the Barley Stripe Mosaic Virus Xingjiang strain ( BSMV-Xj ) were established. The subclasses of these monoclonal antibodies were determined by ELISA. Monoclonal antibodies MA2A2 and MA2B3 belonged to the IgG2b and that of MA7D1 and MA7D7 belonged to the IgG2a and IgG1 respectively. Titers of ascites fluid (measured by indirect ELISA) of MA2A2 and MA2B3 are 103 and MA7D1, MA7D7 are 107. All the monoclonal antibodles reacted strongly with BSMV-Xj strain, but only weakly with the BSMV-An strain. The effect of various treatments on the reactions of BSMV-Xj with these four monoclonal antibodies were monitered by ELISA.更多还原