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复方中药注射液对人癌细胞体外杀伤效应及其对细胞周期的影响

The Killing Action of Chinese Herbal Mixture on Human Tumor Cells in Vitro and Its Effect on Cell-Cycle

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【作者】 张永健王耐勤许树旭刘彤

【Author】 Zhang Yongjian Wang Naiqin Dept.of Pharmacology,Beijing Institute for Cancer Research,Beijing

【机构】 河北医学院北京市肿瘤防治研究所药理组内蒙古医学院北京市肿瘤防治研究所药理组 进修生进修生

【摘要】 复方中药注射液(ICHM)对人胃癌细胞 BGC-823和人乳癌细胞 MCF-7均有较强的杀伤作用。IC50分别为0.02g 生药/mI 和0.018g生药/ml。流式细胞分析仪测定表明,ICHM 使 BDC-823细胞停止在 G1期,导致 S 期细胞显著减少。通过3H-TdR 掺入试验观察到,ICHM 能抑制 BGC-823细胞的 DNA 合成。

【Abstract】 Previous studies had shown that injection of Chinese herbal mixture(ICHM)had antitumor and anti-metastatic actions on various transplantable animal tumors.In the present study,the authors observed the obvious killing actions of ICHM on human gastric cancer cell line BGC-823 and breast cancer cell line MCF-7 in vitro.The IC50were 0.02 g crude drug/ml and 0.018 g crude drug/ml respectively.The study of the effect of ICHM on the cell-cycle of BGC-823 cell showed that ICHM decreased the number of cells in Sphase and increased the number of cells in G1 phase significantly. The results indicated that the BGC-823 cells was blocked on S phase by ICHM.Meanwhile.ICHM reduced 3H-TdR incorporation into DNA,the cpm value was much lower than that of the control.

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