【摘要】 研究设计了一段带终止信号的DNA序列,通过DNA合成仪分为四个片段合成。本文介绍经DNA合成仪合成DNA片段后的处理,纯化及连接过程.经测定DNA的连接效率在65％左右。更多还原

【Abstract】 Four designed DNA fragments which contained a sequence for transcr-iptional termination were synthesized by the DNA Synthesizer Model 381 A (Applied Biosystems). The procedures for separation, purification and liga-tion of these synthetic DNA fragments were described. It was estimated that the efficiency of ligation is about 65%.更多还原