【摘要】 <正> Olucha等曾以庚钼酸铵(AHM)代替硝普钠(SNP)做辣根过氧化物酶(HRP)组化反应四甲基联苯胺(TMB)法的稳定剂(TMB-AHM法),不同程度地消除了原TMB法以硝普钠做稳定剂的缺点。但Olucha用的是普通HRP,对此方法显示标记神经元树突的敏感性未做具体分析。本文将TMBAHM法应用于CB-HRP(Choleragen Subunit B Conjugated HRP)逆行追踪研究神经元嫌高尔基树更多还原

【Abstract】 Ammonium molybdate (AHM) was used as a stabilizing agent in the tetramethy 1 benzidine (TMB) reaction of choleragen subunit B conjugated horseradish peroxidase (CB-HRP) neurohistochemistry. In comparison with Mesulam’s TMB method using sodium nitroprusside (SNP) as stabilizing agent, this new stabilizer offers similar sensitivity in regard to visualization of CB-HRP labelled neurons and their extranuclear Golgi-phobic dendrites. The nearly physiological pH (6-8) of the reaction medium demonstrates better preservation of tissue and cell structures of reacted sections and avoids non-specific needle-like crystal formation. Under the electron microscope, the histochemical reaction products can be clearly distinguished, without the usual shrinkage or distention of cellular and subcellular structures. This method was also applied with success to immunocytochemistry through small modifications.更多还原