【摘要】 同源四倍体水稻杂种花药离体培养,建立了一个高频率再生植株能力的花粉无性系A87203 4C-1-5。试验结果表明:一个芽丛培养一个月,芽数增殖频率为150—200倍。芽的发生方式为芽上生芽。若将芽丛剪碎,重新接在 MS 诱导培养基上,愈伤组织重量的增殖率为5.0倍左右。再将愈伤组织转入 MS 分化培养基上,大约有50%愈伤组织块又可分化出芽,并且仍具有高频率再生芽的特性。继代培养30多代(一年多时间)后仍保持旺盛的增殖能力。通过控制光温条件,可迅速再生植株。植株移栽田间,大多数生长正常,少量外部形态变异。细胞学鉴定,6株为三体(2n+1=25)。更多还原

【Abstract】 Results of study on clone A87203 4C-1-5 from anther culture of homologous tetraploid rice were reported.The trials showed that the clone had a bud multiplication rate of 150—200 times, Under stereoscope,buds were differentiating on buds,forming bud masses.It had a callus induction frequency about 5 times when the bud masses were clipped into pieces and cultured on MS medium for dedifferentiation.Buds with the characteristics of high multiplication were able to be produced from about 50% of the calli when they were transferred onto MS me- dium for differentiation.It still maintained a vigorous multiplying ability though it had been subcultured for more than 30 generation during the past 1 year or more.Numerous plantlets were able to be produced by culture under regulated temperature and light conditions.A number of plantlets deriving from the buds had been planted in the fields this year.Most of them were normal,however,few plants had morphological variations,with 6 trisomic(2n+1 =25)plants observed during meiotic division of their pollen mother cells.更多还原