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苏云金杆菌制剂标准化及毒力效价测定——Ⅰ.用菜粉蝶幼虫测定苏云金杆菌毒效

STANDARDIZATION OF BACILLUS THURINGIENSIS PREPARATIONS I.BIOASSAY WITH PIERIS RAPAE

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【作者】 罗绍彬阎建平戴顺英刘娥英张用梅胡名瑞

【Author】 LUO Shaobin YAN Janping DAI ShunyingLIU Erying ZHANG Yongmei HU Mingrui(Wuhan Institute of Virology, Academia Sinica, Wuhan, Hubei)

【机构】 中国科学院武汉病毒研究所中国科学院武汉病毒研究所 武昌武昌武昌

【摘要】 以室内人工饲养的菜粉蝶幼虫为供试虫,用微量点滴定量感染方法,测定苏云金杆菌制剂的毒力效价,结果较为精确,重复性好,操作简便,完成一次测定只需时2天,标准差14.88%。本文为第一部,介绍生测程序、感染方法和5批测定结果。

【Abstract】 As the first part of a series of reports, this .paper describes the bioassay procedures, methods of infection, and results of 5 bioassays. Larvae of P. rapae reared on artificial diets were infected with the Bt preparations by micro-drop topical application method, to determine the virulence of the bacterial insecticide. The bioassay could be completed in only 2 days, with 14.88% of standard deviation for the results. The method was found to be precise, easy to implement and statistically reliable.

【基金】 中国农科院生防室主持的75-03-03-01项目
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