【摘要】 采用纯化的抗卫氏并殖吸虫成虫抗体进行双抗体夹心HLISA,检测并殖吸虫病人体内特异的循环抗原。检测累计阳性率82.4％(75/91),假阳性率为4.9％(3/61)。其中感染3个月以上的病人血清测得阳性率为51.9％(14/27),感染不足3个月的急性期病人阳性率为85.9％(55/64)。表明此法对并殖吸虫病,尤其是急性期的诊断具有价值。此外还比较了斑点ELISA法与双抗体夹心ELISA法检测循环抗原的效果,发现斑点ELISA法检出率不低于双抗体夹心ELISA法。而且更为简便、省材,在并殖吸虫病诊断和流行病学调查中具有推广应用意义。更多还原

【Abstract】 The specific circulating antigens ( CA) in paragonimiasis can be detected by ELISA with purified antibody which is against adult paragonimus wetermani. Meanwhile a dot-ELISA was conducted to compare with it.The positive rate o’f ELISA in 91 paragonimiasis cases was 82.4% with a false positive rate of 4.9% in 61 healthy individuals. The positive rate of the acute paragonimiasis cases infected within 3 .months was 85.9%, and the positive rate, was 51.9% in the patients infected for over three months. ,f t seems that the detection of CA in diagnosis of paragonimiasis was more nsefnl in the early infection than in the late onel. The positive rate of dot-ELISA was just as same as that of ELISA, so it may be USeFUl in thediagnosis and the epidemiological investigation.更多还原