【摘要】 为了对包虫病的免疫诊断和免疫预防提供有价值的单克隆抗体,通过对骨髓瘤细胞SP2/0与用细粒棘球蚴囊液免疫的BALB/c小鼠脾细胞融合试验及多次筛选、克隆,建立了分泌单克降抗体的细胞株NIA₂。用ELISA及免疫电镜等方法检测证实该细胞株有特异性。其培养上清分泌抗体滴度为1:2560,注入同系小鼠腹腔,诱生肿瘤和滴度为1:163840的腹水。免疫球蛋白鉴定表明是IgG₁,未见对囊虫的阳性反应。该杂交瘤细胞株经组织培养传代10个月,分泌抗细粒棘球绦虫抗体性能稳定。更多还原

【Abstract】 In order to produce specific monoclonal antibodies for immunodiagnosis and immu noprophylaxis in hydatid disease, a monoclonal antibody cell line NIA2 secreting specific antibodies was established, through fusion of myeloma cells SP2/0 and spleen cells of BALB/c mice immunized with hydatid fluid from a local (Ningxia) patient and repeated screenings and clonings. The specificity was assayed via ELISA, immunoprecipitating and ultramicroscopy. Antibody titer of 1:2560 was obtamed in the supernatant of tne culture medium by ELISA and a high titcr of 3:163840 in seeded ascitic fluid of mice of the same isolate. Immunoglobulin subclass of the NIA2 secreting antibody was deter-mined to be IgG1 which showed no cross reaction to Cysticercus cellulosae. Cloned cell lines from the hybridomas were found consistent in secreting anti-Echinococcus gronulosus antibody through serial passage in tissue culture for ten months.更多还原