【摘要】 作者以人宫颈癌DNA经二轮转染NIH/3T3细胞,得到明显转化灶,将转化细胞注入裸鼠,形成纤维肉瘤。经分子杂交证明,二轮转化细胞均可检测到人顺序,说明人顺序巳转移至小鼠细胞内,并呈恶性转化。将转化细胞及裸鼠肿瘤DNA经Bam HI酶切后,与³²P标记的Ha-ras探针杂交呈6.6kb的专一区带,说明人宫颈癌癌基因与Ha-ras具有同源顺序。更多还原

【Abstract】 DNAs were extracted from human primary cervical carcinoma and were transfected into NIH/3T3 mouse cells by calcium phosphate precipitation method to induce the transformed foci in culture. The human sequences had been detected in the DNAs from the primary and secondary cycle of transformed foci cells with human Alu sequences probe. These implied that human transforming sequence had been integrated into transformed cells and these transformed loci cells were inoculated subcutaneously into nude mice to induce fibrosarcoma in vivo. These results indicated that transforming gene（oncogene）was presentedin DNA of human cervical carcinoma, and transferred into mouse cells to induce malignant transformation in vitro and in vivo.Thus, the DNA samples from the secondary loci cells were identified with the oncogene probes of Ha-ras, K-ras and N-ras. The foci DNAs were digested with BamHI restriction enzyme and hybridization with ³²p labelled Ha-ras oncegene probe. The results revealed that the 6.6kb BamHI positive zone was in DNA of cervical carcinoma transformed cells. It showed that the transforming gene of cervical carcinoma is homologuous to Haras oncogene, whereas other oncogenes such as K-ras, N-ras are negative.更多还原