【摘要】 实验成功地从鸡肫中提纯了肌动蛋白并制备了相应的抗肌动蛋白抗体。应用间接免疫荧光技术,观察了照射前后细胞浆微丝分布模式的变化。未经辐照的细胞可见到明亮的膜下荧光,部分呈连续的环状,部分为不连续的“C”形荧光。辐照后细胞膜下荧光消失。如果照后经一段时间培养后再进行观察,则这种变化可以得到恢复。此外,我们还应用DNA酶抑制法,定量地测定了辐照前后细胞内肌动蛋白的含量。结果表明,辐照前后细胞胞浆肌动蛋白的含量及G/F肌动蛋白含量比值均未见到明显的变化。因而提示,电离辐射造成细胞运动功能异常的主要原因是由于电离辐射破坏了膜下微丝的网状结构,使肌动蛋白在细胞内分布异常的缘故。更多还原

【Abstract】 Actin was successfully extracted and purified from chicken gizzard and anti-actin antibody was also obtained. The indirect immunofluorescent technique was chosen for investigating microf ilament distribution in unirradiated and irradiated Raji cells. The results showed that fluorescent rings or crescents were seen at the periphery of cells. The positive rate of the fluorescent patterns was lower after irradiation. This alteration was restored in 24 hours. To determine the actin content in cells the deoxyribonuclease I inhibiting test was adopted. However, no difference in actin content and in G/F ratio between unirradiated and irradiated cells was found. The results suggest that irradiation injures only the cortical meshwork of microfilaments beneath the plasma membrane .This is probably one of mechanisms by which irradiation makes the motor function of cells abnormal.更多还原