【摘要】 本文报告了检出小鼠末稍血正染红细胞(NCE)微核的方法。雄性小鼠分为3组;环磷酰胺(CP)组、丝裂霉素C(MMC)组和骨髓对照组。经腹腔注射给予受试物。试验持续5周。给予CP和MMC的动物在经5次注射后,末稍血中NCE微核率显著增加,微核率随时间的延长而增加。在处理的第5周的微核率:CP(22mg/kg)组为19.3/1000NCE(N=6),MMC(0.5mg/kg)组为29.7/1000NCE(N=6),对照组为1.83/1000NCE(N=6)。用CP(22mg/kg)和MMC(1.0mg/kg)处理的小鼠的骨髓嗜多染红细胞微核率分别为2—3.5和2.5—4.5/1000PCE。在处理后第5周,正染红细胞微核率比第1周高3—6倍。因为有微核的红细胞在循环血中大约存在一个月。所以在此时间内由于多次处理所产生的微核率是相加的,它比传统的微核试验方法更为敏感。更多还原

【Abstract】 The authors reported a method of detecting micronuclei in peripheral normochromatic erxthrocytes(NCE)in mice. Male mice was divided into 3 groups: cyclophosphamide(CP) group, mitomycin(MMC)group and bone marrow control group. The treatment continued for 5 weeks. The rate of micronucleated NCE in peripheral blood was increased significantly after only 5 injections for each of the two chemicals. The micronuclens rate increased consistently with time. Following rates were observed at 5-th week: CP(22 mg/kg), 19.3/1000NCE(N=6); MMC(0.5mg/kg). 29.7/1000NCE (N=6) and control 1.83/1000NCE (N=6), while those for polychromatic erythrocytes (PCE) in bone-marrow of mice treated by CP (22mg/kg) and MMC (1.0mg/kg)are 2—3.5 and 2.5—4.5/1000PCE, resp. The rate of micronucleated NCE at 5-th week after begining of treatment was 3 to s fold higher than that of administration for one week. Since micronucleated erythrocytes survive in the peripheral circulation for about 1 month, micronucleus rate resulting from repeated treatment is additive, it is more sensitive than the traditional micronuclei test method.更多还原