【摘要】 草鱼出血病病毒感染鱼肾（Clk）单层细胞后,产生明显的细胞病变,其繁殖周期为2—3天。病毒增殖的上升期在感染后的12—48小时。28℃下,当感染复数（Multiplicityof Infection简称MOI）为0.05PFU/cell时,病毒滴价可达1.78×10¹¹PFU/ml,不同温度（25℃、28℃、30℃）下通气培养,测定病毒滴价略有差异。感染病毒的细胞培养液经ELISA检测和电子显微镜观察均为阳性结果,并且能感染健康草鱼。更多还原

【Abstract】 Some biological properties of Grass Carp Hemorrhage Virus （GCHV）-873have been studied in this report. This virus could make grass carp kidney cells（CIK） to produce a unique cytopathic effect. At 28℃ its logarithmic growthphase occured 12 to 48 hr after infection. The virus titer reached 1.78×10¹¹PFU/mlwhen the MOI was approximately 0.05PFU/cell. There were slight differencesin virus titer under incubation of 25℃, 28℃and 30℃ respectively, and thesimilar results in 5% CO₂ or in normal atmosphere. The virus particles inculture fluid of infected cells were further eonfirmed by using ELISA andelectron microscopy, and it could infect healthy grass carp as well更多还原