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单克隆抗体与血卟啉衍生物交联物抗胃癌作用的实验研究

EXPERIMENTAL STUDY OF ANTI-TUMOR EFFECT WITH CONJUGATES OF MONOCLONAL ANTIBODIES AND HEMATOPORPHYRIN DEPIVATIVE

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【作者】 许树旭王耐勤董志伟

【Author】 SX Xu, NQ Wang and ZW Dong (Beijing Institue for Cancer Research,Beijing 10031)

【机构】 内蒙古医学院北京市肿瘤防治研究所北京市肿瘤防治研究所 进修生北京 100034北京 100034

【摘要】 为了提高血卟啉衍生物HPD的光敏效应,减低副作用,将其与抗胃癌McAb3G9和3H11交联。体外实验中,3G9-HPD交联物+照光比单用HPD对胃癌细胞株BGC823的细胞毒效应提高17倍,3H11-HPD交联物提高8.6倍。将两种交联物进行不同比例组合,组合后的杀伤效应未见进一步增高。两种交联物对荷瘤裸鼠的抑瘤效应明显高于各对照组,并且能显著延长荷瘤小鼠的生存期。表明McAb对HPD有导向作用。

【Abstract】 Hematoporphyrin derivative (HPD)is a photosensitizer for use in photodynamic therapy (PDT).In this paper, HPD was conjugated with monoclonal antibodies 3G9 or 3Hll for use against gastric cancer in order to enhance the photosensitive effect and reduce side effect of PDT.The biological activities of the McAb conjugates were demonstrated.The killing effect on BGC823 cells of 3G9-HPD or 3H11-HPD conjugates plus exposure to light showed 17 fold and 8.6 fold greater cytotoxicity,respectively than free HPD at an equivalent HPD concentration in vitro. When 3G9-HPD and 3Hll-HPD were used in combination at 7 : 3, 5 : 5 and 3 : 7 proportion,the cytotoxicity was increased 12.9, 11.8 and 9.4 fold on target cells, respectively. The resuls indicate that the eytotoxicity was not further enhanced by the combination scheme. When tumor-bearing nude mice were treated with the different conjugatcs. Significant inhibition of tumor growth was observed and the survival period of animals was markedly prolonged in comparison with PBS,free HPD and NIgG-HPD treated groups.

  • 【文献出处】 药学学报 ,Acta Pharmaceutica Sinica , 编辑部邮箱 ,1989年06期
  • 【被引频次】8
  • 【下载频次】8
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