【摘要】 COPT是目前国内外学者已公认的血吸虫病血清学诊断方法之一.为便该法更为完善、可行,提高虫卵得量,本研究对COPT抗原制备过程中的有关因素作一较系统的研究,结果提示,(1)在制备COPT抗原时,实验兔感染血吸虫尾蚴后所获虫卵量似主要决定于合抱虫体的数量,所用阳性钉螺每杯不宜少于50只.(2)每只实验兔感染1.000～1.500条尾蚴为宜.(3)无论制备冰冻还是热超声干卵,感染后45天剖杀实验兔收集虫卵,制备抗原较为适宜.(4)雌兔或雄兔对虫卵得量似並无影响,均适宜于制备COPT抗原的实验用兔.本文还对制备过程中的其他有关因素作了进一步的探讨。更多还原

【Abstract】 COPT is one of the available methods for immuno-diagnosis of schistosomi-asis, which has been demonstrated by researchers in our and other countries. In order to standardize the test and increase the amount of ova antigen obtained for COPT, we attempted to study the relative factors systematically in the procedure of preparation of ova antigen. The results showed that 1, the quantity of ova obtained seemed to depend on the number of paired adult worms in the infected rabbit, and the number of infected snails in each beaker should not be less than 50 during experiment. 2, the optimal number of Cercaria to infect one experimental rabbit was 1000?500. 3, the eggs obtained on the 45th day after infection was most suitable for both quantity and quality of either lyophilized ova antigen or heat-ultrasonic ova antigen for COPT. 4, both male and female rabbits were suitable to use as experimental rabbits for preparation of ova antigen. This report also studied the other relative factors in procedure of preparation of COPT ova antigen.更多还原