【摘要】 ¹²⁵I-人超氧化物歧化酶是采用联结法进行标记,先标记羟苯丙酸酯,（Bolton Hunter Reagent）,再将¹²⁵I-B.H.R 与SOD 相联。¹²⁵I-B.H.R 标记率为95%,放射性比度可达0.376 mci/μg,稳定性在两个月以上。¹²⁵I-SOD 联结标记率为50%,放射性比度为3.04μci/μg,用醋酸纤维膜和聚丙烯酰胺凝胶电泳后进行放射性层析扫描,放化纯在95%以上,标记不影响该酶的生物活性和免疫活性,冷冻干燥,-20℃保存两个月不失去免疫活性,放化纯仍在95%以上。更多还原

【Abstract】 Superoxide Dismutase（SOD）from human wasradi（?）iodinated with conjuction method:first the BoltonHunter reagent with ¹²⁵I using a chloramine T method,se-con ¹²⁵I-BHR was conjunction with SOD,Iodinatedeffeiciency of ¹²⁵I-Boltton Hunter reagent was 95%,itsspecific activity was 0.376 mCi/μg and it can be stableover two months.¹²⁵I-SOD was purified by columnchromatography.The conjuncted efficiency was 50%.Itsspecific activity was 3.04μCi/μg.¹²⁵I-SOD showed agood bioactivity and immunoactivity.Freezing anddrying.¹²⁵I-SOD was stored at -20℃.It can be sta-ble over two months.更多还原