【摘要】 结合免疫金银染色(IGSS)法和图象定量分析,观察了不同年龄大鼠脑干中缝核群P-物质(SP)样神经元的衰老变化。结果表明:在中缝核群内,SP 样神经元主要分布于中缝苍白核、中缝隐核、中缝大核和中缝背核内。中缝背核内的SP样神经元从中年即开始丧失,而其它核团的神经元到老年才开始减少(P<0.01)。从幼年到中年,各核团的SP 样神经元的截面积几乎增大一倍;但到老年,仅于中缝苍白核内的神经元还在继续增大(P<0.05),而其它核团没有明显变化。各核团的SP 样神经元的灰度值均呈增龄性升高,提示神经元的SP 含量和活性在衰老进程中逐渐降低。老年组SP 样神经元的形态改变呈现为胞体肿胀,边缘不规整,免疫反应减弱,染色浅淡。更多还原

【Abstract】 The aging changes in substance P(SP)-likeneurons of the brainstem raphe nuclei in young(2months),adult(10 months)and old(24 months)rats were examined combining the immunogold-silverstaining(IGSS)with the image quantitative analyticalstudy.In tie raphe nuclei,SP-like neurons was mainlylocalized in the nucleus raphe pallidus,nucleus rapheobscurus,nucleus raphe magnus and nucleus raphedorsalis.The neurons in the nucleus raphe dorsalis beganto be lost significantly from the adult and the ones in theother nuclei began to be decreased from the old(P<0.01).From the young to the adult,size of the neuronsin the all nuclei almost doubled,in the old,however,only the neurons in the nucleus raphe pallidus continuedto enlarge(P<0.05)and the were no marked changesin the other nuclei.Gray value of the neurons in the allnuclei increased with senescence,it suggested that contentand activity of SP in the neurons decline throughout thewere cell body swelling,irregular edged andpale-immunostaining.更多还原