【摘要】 用提纯的黄瓜花叶病毒（CMV）种传SS-30株和豇豆株（P株）混合免疫BALB/c小鼠,取其脾细胞与小鼠骨髓瘤细胞SP2/0,用BAEKON 2000基因转移仪使其融合。通过2—3次简易、快速的有限稀释,获得6个能持续分泌抗体的杂交瘤细胞株,所获抗体分属IgG和IgG_2a,并能诱导高滴度腹水抗体。间接ELISA试验证明这些单克隆抗体对种传CMVSS-30株、P株和非种传CMV的黄化、日本、山东、香蕉、向日葵和Q等分离物有特异性反应,与C_（?）分离物的反应性较弱,而对花生矮化病毒（PSV）无交叉反应。利用间接ELISA测定了几个单克隆抗体的亲和力常数。单克隆抗体C₇H₃、A₆H₄与G₄G₃、G₄H₃所抗的抗原决定簇相距较远,而C₇H₃与A₆H₄,G₄G₈与G₄H₅为抗同一抗原决定簇的单克隆抗体。更多还原

【Abstract】 Six hybridomas stably producing high titer monoclonal antibodies against CMV have been derived from electrofusion the spleen cells of CMV-ss-30 and CMV-p immunized BALB/c mice with SP2/0 using BAEKON 2000 Advanced Gene Transfer System. Then they were cloned by 2—3 times of simple, efficient, standard-dilution. The monoclonal antibodies were of IgG₁ and IgG₂. subclass. Their specificities were determined by indirect ELISA and show quite strong binding ability against ss-30, p, yellow, Japan, Shandong, banana, sunflower and Q isolates of CMV. The lower binding ability was observed with C₅₉ isolate of CMV. They were not reacted at all with PSV. The affinity cosrant and additivity index of monoclonal antibodies have been determined by indirect ELISA. The C₇H₃ and A₆H₄, G₄G₈ and G₄H₅ have same determinant. The C₇H₃, A₆H₄ and G₄G₈, G₄H₅ might recognized different determinant.更多还原