【摘要】 <正> 将地衣芽孢杆菌(Bacillus licheniformis)749/C染色体上的β-内酰胺酶基因克隆于E.coli质粒(pMB9)上,该质粒经EcoRI重排方法进行分子量消减获得了具有氨苄青霉素抗性(Ampicillin resistance,简称Ap~r)的质粒(pCHT 3 Ap~r),以相同的方法处理枯草杆菌(Bacillus subtilis)的质粒(pUB110)获得了具有卡那霉素抗性(Kanamycin resistance,简称Km~r)的质粒(pCHG19 Km~r)。将pCHT3和pCHG19拼接更多还原

【Abstract】 The shuttle plasmid (p~# GTE5) DNA with secretive function was extracted by the alkali lysozyme method from E. coli RRI strain. Its molecular weight is 4.5 Md and DNA size is 6.9Kb. Restriction fragments of plasmid was obtained by single and double enzymes complete digestion using five different restriction endonucleases. The restriction map of shuttle plasmid (p~# GTE5) was established for the enzymes EcoRⅠ, BglⅡ, pstⅠ, PvuⅡ, and TaqⅠ.更多还原