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中药全蝎抗肿瘤作用初探 Ⅱ 对癌细胞作用观察和有效成分提取
A PRELIMINARY RESEARCH OF THE ANTINEOPLASTIC EFFECTS INDUCED BY BUTHUS MARTENSII OF CHINESE DRUG Ⅱ. observation of the effect on cancer cells and the extraction of its effective composition
【摘要】 用全蝎提取物水溶液,对体外传代培养的人体肝癌细胞(BEL—7402细胞)和人体子宫颈癌细胞(HeLa细胞)作观察。结果:全蝎提取物的水溶液在相当生药10mg/ml时,用药48~72小时内使上述细胞全部脱壁死亡。进而在Hela细胞上证明,相当生药0.1mg/ml时也同样作用;並以此为有效标志,查明530号粗提物为全蝎直接抑杀癌细胞的有效部位。
【Abstract】 The effect of water solution of Buthus Martensii (BM)on cultivated human cancer cells of the liver (BEL-7402 cells) and of the cervix (HeLa cells) was observed. All the cells died in 48-72 hours when BM extract solution was used in a concentration of 10mg/ml corresponding with crude drug. And the same effect on HeLa cells was found when the concentration was reduced to 0.1mg/ml.Taking this as the valid sign, we demonstrated that the cancer cells were strangled directly by the effective part of BM in No.530 roughly extracted mateial.
【关键词】 全蝎;
抗肿瘤药物;
中医药;
肝癌/药物作用;
子宫颈癌/药物作用;
【Key words】 buthus martensii; antineoplastic agent; chinese traditional mediaine; liver neoplasms/DE; cervix neoplasms/DE.;
【Key words】 buthus martensii; antineoplastic agent; chinese traditional mediaine; liver neoplasms/DE; cervix neoplasms/DE.;
- 【文献出处】 皖南医学院学报 ,Mcta Academiae Medicinae Wannan , 编辑部邮箱 ,1989年02期
- 【被引频次】9
- 【下载频次】128