【摘要】 油桐尺蠼核型多角体病毒(BsNPV)的多角体大小为1～1.5μm×0.8～1.6μm。病毒粒子长度为0.31～0.8μm。直径为0.05～0.06μm。在0.1×SSC溶液中测得其基因组DNA T_m值为76.5℃,(G+C)%为55.14%,电镜法测得其DNA分子平均长度为41.3μm,计算出对应分子量为81.36×10~6 daltons。用限制性内切酶混合酶解后琼脂塘凝胶电泳,测出DNA积加分子量为79.36×10~6 daltons(120.23kb)。更多还原

【Abstract】 The range in size of Buzura Suppressaria NPV occlusion body was 1～1.5μm×1～1.5μm with polyhedron shape, and virion was rod-shaped, 0.31—0.34μm in length and 0.05-0.06μm in diameter. Tm of viral genome in 0.1×SSC solution was 76.5℃. The (G+C)% content determined by Tm was 55.14%. The average length of BsNPV DNA determined by electron microscopy was 41.3μm, corresponding to a molecular weight of 81.36×10~8 daltons. The viral genome was examined with restriction endonuclearase, single and double digestion DNA using BamHI/Bgl Ⅱ KpnI/XhoI etc. and analyzed in 0.7～1.0% agarose gel electrophresis, the result of which showed a total moleclar weight of DNA genome of 79.35×10~6 daltons (120.23kb). The length of DNA fragment was calculated by Southern’s formula in computer.更多还原