【摘要】 本实验采用疏水性荧光探针DPH(1,6-diphenyl-1,3,5-hexatriene,1,6-二苯基-1,3,5-己三烯)标记小鼠的淋巴细胞膜,根据荧光偏振值的变化比较正常细胞膜和癌变细胞膜流动性及对外界刺激反应性的差别。实验结果表明癌变淋巴细胞膜的流动性明显增高(P<0.001)。用同样浓度的ConA 10mg/L,PHA 40mg/L刺激后,荧光偏振值的变化幅度和方向也不同于正常细胞。本文还对以细胞膜流动性变化作为癌变指标的可能性作了补充说明。更多还原

【Abstract】 In our experiment,lymphocyte membrane was labelled by DPH fluorescence probe. The rate of rotation of the probe can be measured as the value of fluorescence polarization(PDPH). It will provide us with useful information about the membrane fluidity of lymphocytes. We observed that the F value (unit of lipid fluidity of membrane)of the cancerated lymphocytes is obviously higher than that of normal ones. Furthermore, the F value of cultured leukemic Ts lymphocytes is the highest. In contrast with the normal spleen T-, or mixed lymphocytes, the response of membrane of malignant lymphocytes to the stimulation of ConA or PHA is reflected in the decrease of PDPH value or the increase of F To our surprise, the F of T- lymphocyte from the "615" mouse not injected with tumour cells is also higher than that of the mixed. The question of the membrane fluidity as a diagnostic index is also discussed.更多还原