【摘要】 本文测定了97例正常及不育男性精子及精浆中的乳酸脱氢酶C₄同功酶（LDH-C₄）活性。精浆/精子中该同功酶活性的比值在正常对照、精液常规正常而不育（不育Ⅰ组）、精液常规不正常的不育（不育Ⅱ组）和少精症组中依次为0.528,0.880,2.85和4.01（中位数值）。各不育组与对照组间均差异显著（P<0.005及P<0.0005）。以mU/10⁶精子表示时,不育Ⅰ和Ⅱ组精浆中的LDH-C₄活性大于正常男性;精子中的活性则小于正常男性（P<0.005及P<0.0005）。此外,精子中LDH总活性与LDH-C₄活性之间有良好的正相关（r=0.9593）。更多还原

【Abstract】 A study on lactate dehydrogenase isoenzyme C₄（LDH-C₄） in spermatozoa and seminal plasma was performed with following groups: normal males were refered to control group, infertile men with and without normal semen quality were divided into Inf.Ⅰ and Inf. Ⅱ groups, and oligospermic patients were subjected to the fourth group, respectively. The ratio of LDH-C₄ in seminal plasma to that in spermatozoa was: 0.528 (P₅₀) in control, 0.880 (P₅₀). 2.85(P₅₀) and 4.01(P₅₀) in Inf. Ⅰ, Ⅱ and oligospermic groups, respectively. The difference between normal and the others was significant （P<0.005）. The activities of LDH-C₄ in seminal plasma from Inf. Ⅰ and Inf. Ⅱ groups, expressed as mU/10⁶ sperm, were higher than that of normal males and the activities in spermatozoa from the two former groups were much lower than that of normal.The activity of LDH-C₄ in spermatozoa might become an important biochemical index for estimation of semen quality and male fertility. A good correlation between LDH and LDH-C₄ in spermatozoa was found （r=0.9593）.更多还原