【摘要】 采用间接免疫荧光技术,观察了⁶⁰Co γ线对Wistar 大鼠脾B 淋巴细胞体外照射及其迁移和归巢至同系受体脾脏、肠系膜淋巴结、胸腺和血液等组织的辐射效应。实验表明:（1）体外照射的B 细胞数（%）随照射剂量增加呈依赖性递降（P<0.01）;（2）注后3、24h,经1～8 Gy 受照淋巴细胞迁移和归巢至受体脾脏和肠系膜淋巴结的B 细胞数（%）与对照组相比均有非常明显地下降（P<0.01）,且与体外照射的辐射效应呈平行关系。但归巢至胸腺和再循环迁入血液的B 细胞数（%）与对照组相比无明显差别（P>0.05）,（3）从分布比率提示受照B 细胞继发性迁移和归巢至脾脏的量呈上升趋势,而归巢至肠系膜淋巴结的量则继续减少。更多还原

【Abstract】 The experiments were dealt with the effect of ⁶⁰Co γ-ray irradiation on the sple-nic B lymphocyte traffic and homing to spleen,mesenteric lymph nodes（MLN）,thy-mus,and blood of syngenetic recipient rats.By use of indirect immunofluoresencetest,the results showed: （1） irradiated B lymphocytes demonstrated an exposure-dose-dependent decrease in vitro（p<0. 01）; （2） there was a significant inhibiting effect onB lymphocyte migration into recipient spleen and MLN after 1-8 Gy irradiation atintervals of 3 and 24 hours after intravenous transfer（p<0.01）,——parallel to the ra-diation effect on B lymphocytes in vitro;and （3） in the spleen/MLN ratio,the irra-diated B lymphocytes increased relatively in their distribution to spleen at the expenseof homing to MLN in secondary migration.更多还原