A total gene for a-human atrial natriuretic peptide(a-hANP) has been chemically synthesized by solid-phase phosphoramidite method. The synthetic gene consisted of 96 bp contains the structural gene encoding a-hANP, a glutamic acid codon GAA at 5'end of the gene for enzymatic cleavage of expressed product by endoproteinase Glu-C, a stop signal TGA at 3' end of the structural gene and two restriction site at both ends of the gene. The synthesis of the gene involved enzymatic joining oligonucleotides to form D...