【摘要】 本工作在离体细胞水平观察ZnGl₂对链佐霉素（STZ）诱发的胰岛β细胞损伤的保护作用,并分析其可能的作用机制。结果如下:向培养的胰岛细胞中加入生理盐水和STZ（3mmol/L）,孵育12h后,活细胞数由实验前的70万个/ml降至43.93±1.16万个/ml;将ZnCl₂（0.25、0.5、1.0mmol/L）和相同剂量的STZ一同加入细胞,可不同程度地缓解STZ对胰岛的破坏作用,在含不同浓度ZnCl₂的培养液中活细胞数分别恢复至47.39±0.88,58.06±2.29,67.72±1.48万个/ml,与STZ破坏组相比,分别具有显著差异,并呈量效关系。在给予ZnCl₂（1.0mmol/L）的同时,向细胞中加入蛋白合成抑制剂亚胺环已酮（100μg/ml）,可翻转ZnCl₂的作用,活细胞数由63.17±2.15万个/ml,又重新减至45.77±0.76万个/ml。单独加亚胺环己酮对活细胞数目无明显影响。用³H-亮氨酸掺入实验观察ZnCl₂对胰岛细胞蛋白合成的影响发现,单独给予ZnCl₂（1.0mmol/L）仅使蛋白合成轻微增加,与盐水对照组无显著差异;在给ZnCl₂的同时加入STZ,则蛋白合成明显增多,保护组与STZ破坏组比较,差异显著。上述结果表明,增加细胞内蛋白合成,以加强细胞自身对外来损伤的修复力,可能是ZnCl₂保护胰岛细胞的机制之一。更多还原

【Abstract】 The beneficial effect of ZnCl₂ on islet cells injuried by streptozotocin was observed directly on the cultured cells and its possible mechanism was analysed. The results were as follows: （1） The number of viable cells in culture was reduced from 70×10⁵/ml to 43.93±1.16×105/ml 12 hours after adding normal saline plus STZ （3.0mmol / L）, and this reduction could be alleviated by adding ZnCl₂ （0.25, 0.5, 1.0mmol / L） in varying amounts and a dose-response relationship was found. The number of viable cells was returned to 47.39±0.88×10⁵/ml, 58.06±2.29×10⁵/ml, and 67.72±1.48×10⁵ /ml respectively in the culture with ZnCl₂ of different levels. （2） The effect of ZnCl₂ was reversed when cycloheximide（100μg/ml）, a protein synthesis inhibitor, was added into the cultural medium, and the number of via（?）c cells was again decreased from 63.17±2.15×10⁵ / ml to 45.77±0.76×10⁵ ml. No obvious effect was observed when cycloheximide was given alone. （3） The experiment of ³H-leucine incorporation into islet cells showed that protein synthesis was increased slightly and insignificantly after adding ZnCl₂ （1.0mmol / L）. However, there was a significant increase of protein synthesis in cells when ZnCl₂ （1.0 mmol/ L） was added with STZ （3.0mmol / L）.The results suggest that ZnCl₂ has a protective effect on the damaged islet cells induced by STZ. The increase of protein synthesis may be one of the mechanisms involved in the action of ZnCl₂, which strengthens the repairing ability of islet cells after injury.更多还原