【摘要】 我们以往的工作表明,脑内血管紧张素Ⅱ(AⅡ)作为一种抗阿片物质参与吗啡耐受和电针耐受。本工作探讨在此过程中脑内AⅡ的基因表达是否加速。采用酚抽提法提取大鼠脑组织总RNA,使用人工合成的寡脱氧核糖核酸探针进行打点杂交。放射自显影结果表明,多次皮下注射吗啡或连续数小时电针的大鼠脑血管紧张素原(Ang)mRNA含量明显升高。在IBAS图象分析仪上,测量各杂交点自显影曝光斑的积分光密度值(I.O.D.)表明,连续注射吗啡1d或4d使Ang mRNA分别增高1倍或8倍;电针3或6h使Ang mRNA分别增高5倍或13倍。说明大鼠经3—4h吗啡或电针处理,即可引起在转录水平上脑内Ang基因表达加强。更多还原

【Abstract】 The aim of the present study was to explore whether the expression of angiotensinogen (Ang) gene was accelerated during the development of morphine tolerance or electrocupuncture tolerance. The total rat brain RNA was extracted by phenol, and dot blot hybridization with synthetic Ang oligo-DNA was adopted for measuring Ang mRNA. Autoradiographic examination revealed a marked increase in Ang mRNA in rats receiving multiple injections of morphine or continuous electroacupuncture (EA) stimulation for several hours. The integrated O.D. (I.O.D.) of the hybridized dots revealed a 1-fold and 8-fold increase in Ang mNRA in rats receiving morphine injection for 1 and 4 days, respectively, and a 5-fold and 13-fold increase in rats receiving 3 and 6h EA stimulation, respectively. The results suggest that transcription of Ang gene in rat brain can be accelerated within 3—4h by continuous morphine or EA stimulation and that a longer lasting stimulation resulted in a stronger gene expression.更多还原