【摘要】 用超速离心纯化的乙型脑炎病毒抗原,建立了间接ELISA方法检测乙脑IgG抗体。该法敏感性优于常规的血凝抑制试验(HI)和间接免疫荧光法(IFA)方法,特异性为93.3％。用此法调查了上海市666份人群血清中的乙脑抗体。结果<10岁儿童阳性率为50％左右,市区和郊区接近:10～19岁市区和郊区阳性率分别为30.1％和77.8％;20～60岁者市区阳性率为65.9～89.7％,而郊区几乎全部阳性。对不同年龄,市区和郊区抗体水平差别的原因及其与接种乙脑疫苗和乙脑疫情的关系进行了讨论。更多还原

【Abstract】 With purified Japanese encephalitis (JE) virion, an indirect ELISA was developed to detect antibody of IgG class against JE. This method was more sensitive than conventional HI and IFA, and its specificity was 93.3%. 666 serum specimens of various age groups from both urban and rural areas of Shanghai were assayed by ELISA. In children under 10 years of age, about 50% showed JE antibody with no difference between rural and urban areas, whereas in teenagers the positive rates were 30.1% in city area and 77.8% in the countryside. Of urban residents aged from 20 to over 60, 65.9-89.7% were seropositive for JE antibody while in rural dwellers of the same age almost all possessed JE antibody. Factors that may influence the fluctuation of JE antibody prevalence rates in different ages and areas are discussed. These factors include JE vaccination and JE prevalence pattern in the past.更多还原