【摘要】 应用淋巴细胞杂交瘤技术制备出5株抗羊垂体催乳素单克隆抗体。以羟磷灰石柱层析纯化一种单克隆抗体2D2(属IgG_2²)。与环氧氯丙烷活化的Sepharose 4B偶联制备免疫吸附层析柱。以此免疫吸附柱从羊垂体粗提液中简单快速地纯化了催乳素。更多还原

【Abstract】 Five monoclonal antibodies against ovine prolactin (oPRL) were obtained by lymphocyte hybridoma technique. One of these monoelonal antibodies, 2D2, with lower affinity was purified by hydroxylapatite chromatography. 2D2 was coupled with Sepharose 4B by epiehlorohydrin to form immunosorbent colum, which was used for purification of oPRL from curde preparation with a single-step procedure.更多还原