【摘要】 本文以16个小苍兰品种的花蕾为材料,外植体在MS添加BA 2mg/L,NAA 0.2mg/L和3％蔗糖的培养基上诱导愈伤组织并形成不定芽。继而转入MS添加BA0.2mg/L,NAA0.02～1.0mg/L和3％蔗糖的培养基上促成不定根的分化并形成完整小植株。结果表明:所有接种材料均能诱导出愈伤组织和形成不定芽。出愈率范围为36.1～86.7％,不定芽分化率为2.3～86.4％。更多还原

【Abstract】 The flower buds of 16 cultivas of Freesia were used as experimental meterials. The explants were planted on MS medium supplemented with BA 2mg/L, NAA 0.2mg/L and 3％ sucrose to induce callus and to form adventitious buds. The adventitious buds were transferred into MS medium added BA 0.2mg/L, NAA 0.1-0.02mg/L and 3％ sucrose to produce adventitious roots and to form whole plantlets. The results indicated that all meterials planted MS midium can form callus in the range of 36.1-86.7％ and adventitious buds in the range of 2.3-86.4％.更多还原