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血清刀豆素A结合蛋白的检测及其临床意义
MEASUREMENT OF SERUM ConA-CONJUGATED PROTEIN AND ITS CLINICAL SIGNIFICANCE
【摘要】 将外源性凝集素ConA与血清中糖蛋白相结合,在电泳上可形成峰,根据峰的高低可测定血清中所谓ConA结合蛋白含量。正常人血清中ConA结合蛋白为95.16±14.54u/dl,慢性肝病时降低,肝细胞性肝癌时升高。本法简便,灵敏度高。初步显示测定血清中该种蛋白有助于肝病的鉴别诊断。
【Abstract】 Concanavalin-A(ConA), a kind of lectin, when combined with the serumglucoprotein, may form a peak in the electrophoresis. According to the heightof the peak, we are able to detect the amount of ConA-conjugated protein. Innormal individuals, the serum level is 95.16%±14.54u/dl, It decreases in chro-nic liver diseases and increases in hepatocellular carcinoma. This method isboth simple and sensitive. The assay for this kind of serum protein is prelimi-narily shown to be helpful in the differential diagnosis of liver diseases.
【关键词】 火箭亲和电泳;
刀豆素A结合蛋白;
诊断;
【Key words】 rocket-affiniation electrophoresis; ConA-conjugated protein; diagnosis;
【Key words】 rocket-affiniation electrophoresis; ConA-conjugated protein; diagnosis;
- 【文献出处】 南通大学学报(医学版) ,Journal of Nantong University(Medical Sciences) , 编辑部邮箱 ,1989年02期
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