【摘要】 明确乳腺癌患者是否存在早期血行转移具有理论和实际意义,目前的各种检查方法尚难以完成。本文首次应用上皮膜抗原、角蛋白单克隆抗体检测乳腺癌患者骨髓中的微小转移取得了成功。实验方法采取敏感的卵白素-生物素-过氧化物酶复合物(ABC法)这一较新的免疫组化技术。对病人在手术麻醉状态下行多部位骨髓穿刺,标本用Ficoll分层离心以集中有核细胞和可能存在的瘤细胞。已完成10例病人,其中4例找到了阳性的肿瘤细胞,为乳癌的早期诊断和治疗开辟了新径。更多还原

【Abstract】 An immunohistochemical(ABC)method was used from multiple bone-marrow sites in patients with breast cancer.Tumor cells were detected in the bone-marrow by the monoclonal antibodies of epithelial membrane antigen(EMA),keratin,when other conventional techniques did not reveal distant metastasis.We conclude that it is a useful method for the earlier diagnosis of the metastases of breast cancer and the further treatment for the patients.更多还原