节点文献
果胶酶的分离提纯及某些性质研究
Studies on the Purification and Charactarization of Peptinase
【摘要】 本文报导了一种制备高纯度果胶酶的方法,用SDS-聚丙烯酰胺凝胶电泳法测得其分子量为32000,用等电聚焦电泳法测得其等电点为pH8.0~8.2,用圆二色谱测得此酶呈无规则卷曲构象。
【Abstract】 The paper reports a method to prepare peptinase with high purity.The molecular weight of the peptinase is 32000 determined by polyacrylamide gel electrophoresis. The isoelectric point of the enzyme is at pH8.0~8.2 measured by the method of electrofocusing. The CD spectrum showed that the peptinase is in the conformation of freedom coil.
- 【文献出处】 吉林大学自然科学学报 ,Journal of Jilin University , 编辑部邮箱 ,1989年02期
- 【被引频次】10
- 【下载频次】449