【摘要】 本文研究10以℃,4以℃,-4℃和-196℃对藻胆体的室温和液氮温度荧光发射光谱的影响。根据F686和F666,F686和F655相对荧光强度的比值和室温荧光峰的波长位置来判断藻胆体的解离程度和完整性。研究结果表明,在10——4℃温度范围内,藻胆体的解离随着温度的降低而加强;藻胆体在-196℃下很稳定;在8个月内未发生解离。更多还原

【Abstract】 The room temperature and liquid nitrogen temperature fluorescence emission spectra of phycobilisomes stored at 10℃, 4℃, - 4℃, and - 196℃ were determined once every seven days. The intactness and dissociation of phycobilisomes were identified according to the ratios of the relative fluorescence intensity of F686 to F666 and F655 in liquid nitrogen temperature fluorescence spectra, and the wavelength locations of room temperature fluorescence emission maxima.1. Effects of low temperature on liquid nitrogen temperature fluorescence emission spectra.The liquid nitrogen temperature fluorescence spectrum of control phycobilisomes had only one fluorescence emission band, it was located at 686 nm. There were three fluorescence emission bands in that of dissociated phycobilisomes, they were located at 655 nm, 666 nm and 686 nm, characteristic of C-phycocyanin, allophycocyanin and allophycocyanin-B respectively. In the liquid nitrogen temperature fluorescence emission spectra of phycobilisemes stored at 10℃, 4℃ and - 4℃, 655 nm and 666 nm fluorescence were increased as storage temperature was decreased and storage time was longer. The liquid nitrogen temperature fluorescence emission spectrum of phycobilisomes stored at - 196℃ for 8 months was the same as that of control phycobilisomes.2. Effects of low temperature on the wavelength locations of room temperature fluorescence emission maxima.The maximum of room temperature fluorescence of control phycobilisomes (stored at room temperature for 0 day) was located at 678 nm; those of phycobilisomes stored at 10℃ were at 677 nm, 676 nm and 675 nm, at 7th, 21th and 35th day respectively; those of phycobilisomes stored at 4℃ were at 677 nm, 675 nm and 673 nm; and those of phycobilisomes stored at- 4℃ were shifted to 676 nm, 674 nm and 671 nm. The wavelength of room temperature fluorescence emission maximum of phycobilisomes is shorter, the dissociation of phycobilisomes is greater. The room temperature fluorescence emission maximum of phycobilisomes stored at- 196℃for 8 months wasn’t changed, it was still located at 678 nm.Our research results indicated that the dissociation of phycobilisomes increased as storage temperature decreased from 10℃ to -4℃. The phycobilisomes stored at - 196℃ were stable.更多还原